ELEMENTAL ANALYSIS OF PRECIPITATES FORMED IN NUCLEI BY ANTIMONATE-OSMIUM TETROXIDE FIXATION

Nuclear Precipitates in Pyroantimonate-osmium Tetroxide-fixed Tissues

Recent biochemical studies indicate a significant role for sodium ions in nuclear metabolism. Protein and nucleic acid syntheses in the nucleus depend upon sodium, apparently through a stimulating effect of this cation on the transport of amino acid, purine, and pyrimidine into the nucleus (1). The nuclei of avian erythrocytes and calf thymocytes appear to contain higher sodium concentrations t...

Oxidation of tertiary silanes by osmium tetroxide.

In the presence of an excess of pyridine ligand L, osmium tetroxide oxidizes tertiary silanes (Et(3)SiH, (i)Pr(3)SiH, Ph(3)SiH, or PhMe(2)SiH) to the corresponding silanols. With L = 4-tert-butylpyridine ((t)Bupy), OsO(4)((t)Bupy) oxidizes Et(3)SiH and PhMe(2)SiH to yield 100 +/- 2% of silanol and the structurally characterized osmium(VI) mu-oxo dimer [OsO(2)((t)Bupy)(2)](2)(mu-O)(2) (1a). With...

Fixation of lung for electron microscopy. Fixation of fresh frozen sections with osmium tetroxide.

Actin filament destruction by osmium tetroxide

We have studied the destruction of purified muscle actin filaments by osmium tetroxide (OsO4) to develop methods to preserve actin filaments during preparation for electron microscopy. Actin filaments are fragmented during exposure to OsO4. This causes the viscosity of solutions of actin filaments to decrease, ultimately to zero, and provides a convenient quantitative assay to analyze the react...

Fixation of Neural Tissues for Electron Microscopy by Perfusion with Solutions of Osmium Tetroxide

This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic le...